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KMID : 0644120100230020038
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Journal of Oriental Gynecology
2010 Volume.23 No. 2 p.38 ~ p.56
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Effect of Foeniculi Fructus on the Ovarian Function and Gene Expression of Caspase-3, MAPK and MPG in Female Mice
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Jeon Mi-Hye
Park Young-Sun
Kim Dong-Chul
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Abstract
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Purpose: This study was designed to evaluate the effect of administration of Foeniculi Fructus on ovarian functions and differential gene expressions related cell viability such as caspase-3, MAPK and MPG in female mice.
Methods: We administered the Foeniculi Fructus to 6-week-old female CF-1 mice for 4, 8, 12 days. After administration of Foeniculi Fructus with 0.1, 1, 10, 100 ㎎/㎖ concentration in the comparison of control group with 0 ㎎/㎖, we observed the mean number of total ovulated oocytes and the number of morphologically normal oocytes. After entosomatic fertilization, we observed the rate of fertilized 2-cell embryos to blastocyst stage in vitro. Also we chose the caspase-3 for cell apoptosis, MAPK and MPG genes for cell viability and DNA repair by RT-PCR.
Results: 1. In case of 4, 8, 12day administration of Foeniculi Fructus with 0.1, 1, 10, 100㎎/㎖, the mean number of total ovulated oocytes and the number of morphologically normal oocytes were increased in the comparison of control group. 2. In case of 4, 8, 12day administration of Foeniculi Fructus with 0.1, 1, 10, 100㎎/㎖, the rates of blastocyst formation from 2-cell stages were increased in the comparison of control group. 3. In case of 4, 8, 12day administration of Foeniculi Fructus with 0.1, 1, 10, 100㎎/㎖, the gene expression of caspase-3, MAPK, MPG didn’t show significant result in the comparison of control group.
Conclusion: This study shows that Foeniculi Fructus has significant effects on the increase of the function on ovulation and embryonic development of female mice. But this results have nothing to do with caspase-3, MAPK and MPG genes. So we need a further study for which genes are related to the activation of reproductive functions of Foeniculi Fructus.
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KEYWORD
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Foeniculi Fructus, Ovarian function, Embryonic development, caspase-3, MAPK, MPG
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